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QC

Quality control was performed on each processing stage. The final QC metrics for the aligned BAM files are shown.

Summary: Good and relatively uniformly mapped data

Load data

[1] TRUE
 [1] A A A B B B C C C D D D
Levels: A B C D

Correlation matrix

Dendrogram

Principal Components Analysis

  PC1 PC2 PC3 PC4 PC5
Standard deviation 20.22 12.59 5.992 4.875 4.499
Proportion of Variance 0.5575 0.2164 0.04897 0.03242 0.02761
Cumulative Proportion 0.5575 0.7739 0.8228 0.8552 0.8829

Helper functions

Comparison

Differentially espressed genes (DEGs) detected at False Discovery Rate (FDR) 0.1. See the total number in the table’ annotations. Top 50 or less DEGs are visualized.

Legend for gene lists: “Geneid”, “symbol”, “description” - gene annotations; “Length” - length of a gene in bp; “logFC” - log fold change; “logCPM” - average Counts Per Million, log2; “LR” - log-likelyhood; “P.Val”/“adj.P.Val” - non-/FDR-adjusted p-value.

DEGs_1: Compare the scramble control without erythroid differentiation to the knockout without erythroid differentiation

DEGs_2: Compare the scramble control with erythroid differentiation with the knockout with with erythroid differentiation

Functional enrichment analysis

Legend: “database” - source of functional annotations, “category” - name of functional annotation, “pval” - unadjusted enrichment p-value, “qval” - FDR-adjusted p-value, “genes” - comma-separated differentially expressed genes enriched in a corresponding functional category, “direction” - UP/DN, an indicator whether genes are up- or downregulated.